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Published in Crop Sci 9:30-33 (1969)
© 1969 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Influence of Naphthaleneacetic Acid and Phosphate Stress on Uterotropic Activity in Trifolium medium L.1

L. M. Gourley, W. F. Keim and Martin Stob2

A single application of naphthaleneacetic acid (NAA) in concentrations of 0.46 or 0.91 x 10–3 M produced a 20 to 25% increase in the uterotropic activity of forage produced by two genotypes of zigzag clover, Trifolium medium L. An application of 1.37 x 10–3 M did not raise uterotropic activity over that of the control. The auxin-treated forage was more lush and had a lower percent dry matter than the untreated plants.

Phosphate stresses, created by varying the amounts of phosphate in the nutrient solutions supplied to two genotypes of zigzag clover in sand culture, failed to increase uterotropic activity. A significant increase in yield of dry matter was observed along with a significant decrease in percent dry matter as the level of P was raised from 0 to 16 to 32 ppm.

Key Words: Estrogenic activity • Zigzag clover

1 Contribution from the Departments of Agronomy and Animal Sciences, Purdue Agricultural Experiment Station, Journal Paper No. 3378. Part of a thesis presented by the senior author in partial fulfillment of the requirements for the M,S. degree. Supported in part by Public Health Service Research Grant No. U.L 00279, National Center for Urban and Industrial Health.

2 Graduate Research Assistant in Agronomy, Professor of Agronomy, and Professor of Animal Sciences, respectively.

Received for publication May 29, 1968.




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