HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kleinhols, A. Articles by Haskins, F. A. Search for Related Content PubMed Articles by Kleinhols, A. Articles by Haskins, F. A. Agricola Articles by Kleinhols, A. Articles by Haskins, F. A.

Mutation Induction in Melilotus Alba Annua by Chemical Mutagens¹

Hydroxylamine (HA), 2-aminopurine (AP), 5-bromouracil (BU), and ethyl methanesulfonate (EMS) were tested for mutagenic activity on seeds of Melilotus alba annua. Mutants were classified into the following categories: o-hydroxycinnamic acid (o-HCA) content and ß-glucosidase activity, chlorophyll deficiency, dwarf growth habit, and miscellaneous morphological variations. Due to difficulties in classification, the o-HCA and ß-glucosidase category and the dwarf category were unreliable for determining mutation rates. Based on the chlorophyll-deficient and miscellaneous morphological categories, mutation rates for HA, AP, and BU treatments did not differ from the control rates, but EMS at 0.003M and 0.004M concentrations was highly effective in inducing mutations in these categories. Mutation rates based on chlorophyll deficiency were somewhat higher and more sensitive to EMS concentration than were mutation rates based on the miscellaneous category. Large numbers of chlorophyll, dwarf, and morphological variants, several more vigorous than the controls, were isolated from the EMS treatments, but no new o-HCA or ß-glucosidase mutants were obtained.

Key Words: sweetclover • ethyl methane-sulfonate

² Formerly Instructor in Agronomy, University of Nebraska (now Assistant Professor of Agronomy and Genetics, Washington State University, Pullman, Wash.), Research Geneticist, Crops Research Division, ARS, USDA, Lincoln Nebr.; and Bert Rodgers Professor of Agronomy, University of Nebraska, Lincoln, respectively.

Received for publication March 23, 1968.

This article has been cited by other articles:

				K.P. Vogel, H.J. Gorz, and F.A. Haskins Registration of N30-N56, N741, N743, N745, N747, U362, U363, U367, U369-U374, U389-U394, U396-U398, and U500 Sweetclover Genetic Stocks Crop Sci., June 24, 2005; 45(4): 1675 - 1678. [Full Text] [PDF]