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Green leaves of annual ryegrass, Lolium multiflorum Lam., with active pustules of crown rust, Puccinia coronata Cda., uredospores were stored from May to July in a refrigerator at 5 to 10 C. They yielded sufficient inoculum for artificial epiphytotics. The technique reduced the time needed for reproducing the disease, and it succeeded in developing 'Magnolia' annual ryegrass, highly resistant to crown rust.
Key Words: spore storage rust spores rust resistance annual ryegrass crown rust
2 Agent, Research Agronomist, Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, and Mississippi Agr. Exp. Sta., State College, Miss. 39762.
Received for publication April 13, 1968.
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