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Published in Crop Sci 8:506-507 (1968)
© 1968 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Germination of Oat (Avena Byzantina C. Koch) Pollen on Artificial Media1

A. T. Wallace and P. Karbassi2

Freshly collected oat pollen was germinated in Petri dishes on media containing 13 g sucrose, 1.7 g raffinose, 20 mg boric acid, 25 mg calcium nitrate, 3 g bacto-agar, and 85 ml double-distilled water. Germination is improved if the dishes containing the media are "aged" 2 to 10 days in a refrigerator. The pollen germinates within 1 to 2 minutes after it comes in contact with the media. Within another 5 to 6 minutes the tubes usually have grown to their maximum length, 125 to 150 microns, at which time the ends burst and exude their contents. Modifying agents proved to be ineffective in preventing the bursting.

Key Words: Pollen Germination • Pollen Tubes • Gramineae

1 Contribution from Florida Agricultural Experiment Station, Gainesville, Florida 32601. Journal Series No. 2919. Supported in part by AEC Contract No. AT- (40-1)-3092. ORD-3092-16. Recieved Feb. 23, 1968.

2 Professor and Head Plant Science Section and Research Assistant.

Received for publication February 23, 1968.




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