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Published in Crop Sci 8:493-495 (1968)
© 1968 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Non-Translocation of Temperature-Induced Self-Compatibility Substances(s) in Alsike Clover, Trifolium Hybridum L.1

C. E. Townsend2 and R. E. Danielson3

Air temperature had a marked effect but soil temperature had no effect on the self-compatibility response of tetraploid alsike clover, Trifolium hybridum L., clone 3-7B. Flowering stems of propagules of tetraploid clone 3-7B and diploid clones 6-5 and 7-1 were arranged to form two groups per propagule. One group was subjected to an air temperature of 32 C and the other to an air temperature of 23 C. Thus the flowering stems of each propagule were exposed to two air temperatures simultaneously. Percentage of florets setting seed for such propagules was dependent upon the air temperature at which the flowering stems were held. At 23 C the propagules were self-incompatible, but at 32 C the compatibility reaction changed to one of considerable self-compatibility. We concluded that the substance (s) responsible for the change in the compatibility reaction was not translocated from one flowering stem to another on the propagule.

Key Words: Divided Propagule Technique • Temperature Control • Sterility


1 Joint contribution of the Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, and Colorado Agricultural Experiment Station, Fort Collins, Colorado 80521. Scientific Series No. 1280.

2 Research Geneticist. Crops Research Division, ARS, USDA, and Faculty Affiliate, Department of Agronomy, Colorado State University

3 Professor of Agronomy, Colorado State University.

Received for publication March 18, 1968.





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