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Published in Crop Sci 8:47-49 (1968)
© 1968 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Evaluation of Methods for Protein and Oil Determination in Soybean Seed1

W. R. Fehr, F. I. Collins and C. R. Weber 2

Seed density, specific gravity, nuclear magnetic resonance, Kjeldahl, and solvent extraction methods were evaluated for their effectiveness in estimating protein and oil content of soybean seed (Glycine max (L.) Merrill). The Kjeldahl method was superior for direct measurement of protein. Nuclear magnetic resonance was superior to solvent extraction as a rapid, accurate method of oil analysis. Seed density and specific gravity were comparable for estimating protein and oil content under most conditions.

Key Words: Glycine max (L.) Merrill • seed density • specific gravity • nuclear magnetic resonance • Kjeldahl • solvent extraction

1 Joint contribution from the Iowa Agricultural and Home Economics Experiment Station, Ames, Iowa 50010, as Journal Paper No. J-5722, Project 1179 and the Crops Research Division, ARS, USDA, as No. 488 of the U. S. Regional Soybean Laboratory. Part of a thesis submitted by the senior author in partial fulfillment of the requirements for the Ph.D. degree.

2 Assistant Professor of Agronomy, Iowa State University; Chemist, Crops Research Division, ARS, USDA; and Director of Research, Peterson Seed Co., Waterloo, Iowa, (formerly Agronomist, Crops Research Division, ARS, USDA, and Professor, Iowa State University, Ames), respectively.

Received for publication July 7, 1967.




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