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Published in Crop Sci 8:39-41 (1968)
© 1968 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Effects of a Suboptimal Temperature on the Free Amino Acid Composition of Bean Seedlings1

H. R. Knull and E. J. Kinbacher2

Phaseolus vulgaris L. var. ‘Michelite’ was grown at an optimal temperature, 25 C, and a suboptimal temperature, 18 C. Seeds and seedlings were divided into different mmorphological sections at three stages of growth and analyzed for total nitrogen and 20 free amino acids. Significant differences in the free amino acid composition of seedlings at comparable stages of growth were observed between the two temperatures. Before the cotyledons emerged through the growth medium, the plants grown at 25 C were higher in free amino acids. At the time of their emergence through the growth medium, the seedlings grown at the suboptimnal temperature were higher in free amino acids. Asparagine was the most abundant free amino acid detected in all seedlings.

1 Published with the approval of the Director as Paper No.2015, Journal Series, Nebraska Agricultural Experiment Station. Includes a portion of a thesis by the senior author in partial fulfillment of the requirements for the M.S. Degree at the University of Nebraska. This research was partially supported by a grant from the Herman Frasch Foundation.

2 Research Assistant and Associate Professor, Department of Horticulture and Forestry, University of Nebraska, Lincoln, Nebr. 68503.

Received for publication September 28, 1967.




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