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Changes in hardiness and plant constituents associated with hardening were observed in Pawnee winter wheat under constant and under decreasing photoperiod and temperature treatments. Hardening occurred only when the temperature decreased, and was greater under a constant long photoperiod than under a decreasing photoperiod. Least hardening occurred when the photoperiod was decreased and the temperature remained constantly high.
Changes in dry matter, reducing sugar, sucrose, and free amino acid contents of wheat crowns paralleled changes in hardiness. Soluble protein content of crowns was increased by decreasing photoperiod as well as by decreasing temperature.
Key Words: cold resistance sugars amino acids proteins
2 Assistant Professor, Department of Agronomy, Kansas State University, Manhattan, Kans. 66502.
Received for publication February 27, 1967.
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