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Published in Crop Sci 8:1-2 (1968)
© 1968 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Influence of Fertilization on Some Organic Acids in Alfalfa, Medicago sativa L1

J. C. Burns, C. H. Noller, C. L. Rhykerd and T. S. Rumsey2

An established stand of Ranger alfalfa was fertilized with six fertilizer combinations: N, NP, NK, PK, NPK, and NPK with trace elements. Forage samples taken daily at 8:30 a.m. and 2:30 p.m. for 8 consecutive days were measured quantitatively for citric, alpha-ketoglutaric, succinic, malic, oxalic and malonic acids by gas-liquid chromatography. Fertilizer significantly altered the amounts of citric, malic, oxalic, malonic acids and the total acids measured. Morning levels of citric and malonic acids were significantly higher (P<.01 and P<.05, respectively) than afternoon levels, while alphaketoglutaric, succinic, and malic acids decreased from morning to afternoon (P<.01). Significant trends (P<.01) curred in levels of alpha-ketoglutaric, malic, oxalic and malonic acids with advance in maturity.

Key Words: diurnal period

1 Contribution from Purdue University Agricultural Experiment Station, Departments of Agronomy and Animal Sciences, Lafayette, Ind. Journal Paper No. 2812.

2 Graduate Research Instructor of Agronomy (now Research Plant Physiologist, USDA, ARS, and Assistant Professor, Department of Crop Science, North Carolina State University, Raleigh, N. C.); Professor of Animal Sciences and Professor of Agronomy, Purdue University, Lafayette, Ind. 47907; and Research Animal Husbandman, USDA, ARS, Animal Husbandry Research Division, Agricultural Research Center, Beltsville, Md. Acknowledgment is made to International Minerals and Chemical Corporation, Skokie, I11., for providing the fertilizer and to American Potash Institute, West Lafayette, Ind., for providing funds for a portion of the analyses.

Received for publication July 6, 1967.




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