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Only a trace of disease developed where spore concentration of the inoculum was as low as 2,000 spores per milliliter. Disease level increased with concentration up to 442,000 spores per milliliter on the more susceptible clone and up to 1,250,000 spores per milliliter on the less susceptible clone. The optimum temperature range for development of the disease was 20 to 24 C. No symptoms developed at 28 C. Low light intensity, especially during the first 4 days after inoculation, favored disease development. Red clover clones differed greatly in their reactions to different isolates of the pathogen. None of the clones was immune to all isolates, but one was immune to 7 of 18 isolates tested and resistant to the other 11. Isolates of Kabatiella caulivora differed in virulence as well as pathogenicity.
Key Words: disease tests light effects on disease inoculum concentration effects on disease resistance to northern anthracnose races of kabatiella caulivora artificial epiphytotics
2 Research Assistant and Professor of Plant Pathology.
Received for publication May 1, 1967.
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