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Five oil samples from each of 3 kernel positions (base, middle, and tip of ear) of 5 ears each of 12 inbred lines were analyzed by gas-liquid chromatography to determine the effect of kernel position on oil composition. Kernel position had little effect on stearic and linolenic acids. In general, the proportion of palmitic and linoleic acids increased and the proportion of oleic acid decreased in the oil from kernels sampled linearly from the base to the tip of the ear. Kernel position exhibited more consistent and greater influence on oleic and linoleic acids than on palmitic acid. Mp428 and R196 showed no effect of kernel position on oil composition, whereas T202 and Tx39-16 showed marked effects of kernel position on oil composition. Since random sampling of kernels of certain inbreds can produce considerable variation in o11 composition due to the effect of kernel position, it was concluded that in studies in which single kernels are analyzed, sampling should be restricted to the middle portion of the ear. Correlation coefficients showed that the analyses of small oil samples (single kernels) were comparable to large oil samples (oil from six kernels).
Key Words: Corn fatty acids of corn oil gas-liquid chromatography kernel position effects corn oil
2 Associate Agronomist, Georgia Experiment Station.
Received for publication March 31, 1967.
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