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Effect of Nitrogen Fertilization on the Amino Acid Composition and Distribution in Sorghum Grain¹

Effects of three levels of nitrogen fertilization and three locations were studied with three replications of each treatment to determine their relationships to the amino acid content and distribution in sorghum grain. Levels of 17 amino acids were determined in each sample. Protein and 17 amino acids were significantly increased by nitrogen fertilization. Distributions of the amino acids were significantly affected. Proportions of glutamic acid, proline, alanine, isoluecine leucine and phenylalanine were greater in protein of grain grown with highest nitrogen fertilization; proportions of lysine, histidine, arginine, threonine, and glycine were lower. Location significantly affected distribution of certain amino acids which may have been due to a different hybrid at one location or to difference in protein levels of grain grown at different locations, or to both.

Key Words: protein • hybrid • nutritionally essential

² Research Associate and Associate Professor, Department of Grain Science and Industry, and Director, Kansas Agricultural Experiment Station, Kansas State University.

Received for publication November 14, 1966.