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Published in Crop Sci 7:223-224 (1967)
© 1967 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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A Bioassay for Alfalfa Saponins Using the Fungus, Trichoderma viride Pers. ex Fr.1

D. E. Zimmer, M. W. Pedersen and C. F. McGuire2

Extracted alfalfa saponins and leaf meal extracts from alfalfa influenced the growth rate of some fungi more than others. Of seven fungi whose growth rate was studied on media to which different concentrations of extracted alfalfa saponins or Leeben, a commercial saponin, were added, only Trichoderma viride was fonnd highly sensitive at concentrations below 1 mg of saponin per milliliter of medium.

A correlation coefficient of —0.952** was found between the growth of T. viride on 2% potato-dextrose-agar containing leaf meal extracts from 36 F1 and S1 plant families and saponin percentage. This, together with a correlation coefficient of —0.922** between the growth of T. viride on media containing leaf meal extracts from individual alfalfa plants and saponin percentage, suggests the use of T. viride for bioassays for saponins.

Key Words: fungistasis of saponins

1 Cooperative investigations of Crops Research Division, Agricultural Research Service, U.S. Department of Agriculture, and the Utah Agricultural Experiment Station. Published with approval of the Director of Utah Agricultural Experiment Station, Journal Paper No. 604.

2 Research Pathologist, Research Agronomist, Crops Research Division, ARS, and Formerly graduate assistant, Department of Plant Science, Utah State University, presently graduate assistant, Department of Agronomy, North Dakota State University, Fargo, N. D.

Received for publication October 28, 1966.




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