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Preliminary Classification of Polysaccharide Freezing Inhibitors¹

Structure of ice masses which form in the vicinity of the cell walls within living plants can affect survival of hardy winter cereals. Soluble polysaccharides which affect ice structure previously were classified on a basis of equilibrium and kinetic relationships which occur as the solutions are frozen in films on cellulose. A simpler method of classifying cell wall polysaccharides on a relative basis is presented. The method is based on interaction of the polymers with an expanding ice lattice as the liquid solution slides across the ice-liquid interface.

² Research Plant Physiologist, Crops Research Division, ARS, USDA.

Received for publication October 14, 1966.