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Isogenic Lines in American Upland Cotton, Gossypium hirsutum L.: Preliminary valuation of Lint Measurements¹

We backcrossed plants of eight dominant markers recurrently to a highly inbred line to develop a series of isogenic lines. After backcrossing six generations, we evaluated eight agronomic and physical properties of the lint to determine the degree of isogenicity, and to characterize the pleiotropic effect of the individual marker genes. After separating the segregating material by genotype, we evaluated them by comparing the variability between and within genotypes. Analyses revealed residual variability in some lines but not in others after six generations of backcrossing, which indicated progress toward isogenicity but that additional backcrossing was needed. Some markers displayed apparent pleiotropic effects and one displayed linkage.

² Research Geneticists and Research Agronomist, Crops Research Division, ARS, USDA.

Received for publication August 15, 1966.