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Published in Crop Sci 7:1-2 (1967)
© 1967 Crop Science Society of America
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Rating of Alfalfa Varieties for Cold Resistance by Germination in Solutions of Different Osmotic Values1

A. Dovrat and Miriam Waldman2

Seven alfalfa varieties differing in degree of winterhardiness were germinated in water and in 6-atm mannitol solution (500 ml substrate) at 20 C in the dark. The delay in germination was expressed as the ratio between the rate of germination over the period at which most of the slope of the germination curve is near linear in the 0- and 6-atm solutions, respectively. This ratio (R0R0 value) was found to be closely related to the apparent winterhardiness of the varieties.

The test enabled fair separation between 19 and 21 seed lots of ‘Ranger’ and ‘Vernal’ alfalfa, respectively. The lots represented different generations of increase and were derived from a wide latitudal range of seed production areas. However, all were from the same harvesting year.


1 Contribution from The National and University Institute of Agriculture, Rehovot, Israel. No. 921-E. 1965 Series. This work was supported by grant No. FG-Is-133, A10-CR-16, from the U.S. Department of Agriculture.

2 Senior Lecturer and Research Assistant, Department of Field and Vegetable Crops, Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel.

Received for publication August 16, 1965.





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