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Use of Shoot-Root Grafts in Studies of Site of -Hydroxycinnamic Acid Synthesis in Melilotus alba¹

The content of o-hydroxycinnamic acid was measured in shoot-root grafts involving four combinations of Melilotus alba plants of the CuCuBB and cucuBB genotypes. Six portions from each grafted plant were assayed. Results provide evidence that o-hydroxycinnamic acid synthesis occurs in the aboveground parts of sweetclover plants, with limited translocation to the roots. No support was obtained for the view that the root plays an important role in o-hydroxycinnamic acid synthesis.

² Research Geneticist, Crops Research Division. ARS, USDA, and Professor of Agronomy, University of Nebraska, respectively. The technical assistance of John F. Hermanussen and Artur F. Welty is gratefully acknowledged.

Received for publication May 5, 1966.