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Designs to Estimate Effects of Clone Substitution in Alfalfa Synthetics¹

Variations of a 4-factor factorial experiment were developed for estimation of components of variance due to general, 2-, 3-, and 4-line combining ability effects in populations of 4-clone alfalfa synthetics. Applications of the double cross and 4-stage nested mating designs were also discussed. Knowledge of the relative importance of the components would be an aid in planning a breeding program.

² Research Agronomist, U. S. Regional Pasture Research Laboratory, Crops Research Division, ARS, USDA, University Park, Pa. 16802.

Received for publication January 8, 1966.