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Leaf removal from clover seedlings, Trifolium repens L., cultivar ladino, reduced leaf area, dry weight, petiole length, and emergence rate, but increased rate of development (leaflet unfolding and separating). Removing two leaflets from each leaf had little effect on the above leaf growth parameters. Shading of all leaves reduced leaf area of the first leaf, but area of succeeding leaves increased at a rate similar to leaves on the nondefoliated plants. It is hypothesized that growth regulators produced by older leaves may be major factors in the growth and development of leaves immediately after defoliation.
2 Research Agronomist, Crops Research Division, ARS, USDA, Beltsville, Md.
Received for publication December 17, 1965.
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