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The Effect of Hot Water Treatment, X-Ray Irradiation and Mesocotyle Grafting on Cytoplasmic Male Sterility of Maize¹

Treatment of dry and germinated maize seeds in a water bath at 67 C and 56 C, respectively, resulted in death of a majority of the seeds but did not cause plants of a cytoplasmic sterile line to be male fertile. X-ray irradiation, while producing aberrant plants, did not cause reversion from sterile to fertile plants. Mesocotyle grafts between sterile and fertile inbred lines did not result in sterility in the otherwise fertile lines or their progeny. These results do not support the theory that a virus is the causal factor for cytoplasmic male sterility in maize.

² Formerly Graduate Assistant (present address, Department of Botany, University of California, Davis) and Professor, Department of Botany and Plant Pathology, Purdne University, Lafayette, Indiana.

Received for publication February 8, 1966.