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Effect of Kernel Position on Amylose Starch Content; Distribution of Amylose within Corn Endosperm (Zea mays L.)¹

Relative amylose starch content of kernels was determined for three ear zones of inbred, F₁ and F₂ generations of high-amylose corn. Each ear was divided into three relatively equal zones, and the amylose content was determined for each. A significant linear decrease in amylose content was found progressing from the base to the tip of an ear. These results indicate that kernel sampling procedures should be standardized where precision is desired in investigations involving high-amylose starch synthesis. In addition, kernels from each of the three ear zones were degermed and the endosperm tissue was divided into tip, middle, and crown portions. The middle endosperm portion was consistently highest in amylose content within each ear zone.

² Assistant Professor and Instructor, respectively, Department of Field Crops, University of Missouri.

³ Research Agronomist, Crops Research Division, ARS, USDA, and Professor, Department of Field Crops, University of Missouri. Appreciation is expressed to the personnel of the Northern Utilization and Development Division Laboratory, USDA, for conducting the amylose analyses. The research reported herein was supported in part by a grant from the Corn Industries Research Foundation, Inc., New York, N.Y.

Received for publication January 11, 1966.