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Published in Crop Sci 6:271-272 (1966)
© 1966 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Mutagenic and Chromosomal Effects of X-rays and Alkylating Chemicals on Triticum durum Desf. ‘Lakota’1

L. H. Edwards and N. D. Williams2

Seeds of Lakota durum were treated with X-rays, ethylene imine (EI), diethyl sulfate (DS), and ethyl methanesulfonate (EMS). Each chemical mutagen produced chromosomal aberrations in less than 3.0% of the M1 spikes examined; whereas, X-rays produced aber rations in 7.6 to 77.2% of the M1 spikes as X-ray dose increased from 7.5 to 30.0 kr. Treatments with 0.5% EMS and 0.02% DS induced chlorophyll-deficient mutations in 23.33 and 6.94% of the M1 spikes, respectively. The highest mutation rates induced by EI and X-rays were 2.64 and 3.82% mutated M1 spikes, respectively.

1 Cooperative investigations of the North Dakota State University, Fargo, and the Crops Research Division, ARS, USDA. Adapted from a dissertation submitted by senior author in partial fulfillment of the requirement for the Ph.D. degree. Acknowledgment is made for financial support from the National Defense Education Act of 1958. Published with approval of the Director of North Dakota Agr. Exp. Sta. as Journal Article No. 50.

2 Research Geneticists, Crops Research Division, ARS, USDA, Fargo, N. Dak.

Received for publication October 16, 1965.




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