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The use of an embryo marker of commercial value for detecting monoploids of maize is described. This system utilizes a male parent called the Purple Embryo Marker (b pl A C Rnj:Cudu pr Pwr) which produces a deep purple pigment in the embryo and red or purple aleurone color in the endosperm. Kernels of a marked progeny which do not exhibit purple color in the embryo but do have red or purple aleurone pigment are saved for the putative monoploid embryos contained. In this study, nine single crosses of commercial value were pollinated with the Purple Embryo Marker stock. Of the 194,157 kernels classified, it was possible to discard more than 98% before germination, l~rom the kernels with putative monoploids 201 actual monoploids were realized.
2 Research Geneticist, Edward J. Funk and Sons, Inc., Kentland, Indiana; and Director, International Seed Operations, DeKalb Agricultural Association, Inc., DeKalb, Illinois, respectively.
Received for publication September 28, 1965.
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