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Influence of Temperature, Light Intensity, and Photoperiod on the o-Hydroxycinnamic Acid Content in Plant Parts of Sweetclover¹

The influences of telnperature, light intensity, and photoperiod on content of o-hydroxycinnamic acid in various parts of sweetclover plants were studied. Increases in temperature, light intensity, and light duration to which the plants were exposed were generally followed by increases in the amounts of o-hydroxycinnamic acid produced in each of the plant parts. Contents of o-hydroxycinnalnic acid in leaves and stems increased most rapidly and, in plants 25 days or more in age, these parts contributed the overwhelmingp ortion of the total plant content. Percentages of o-hydroxycinnamic acid calculated on a dry-matter basis were highest in leaves, intermediate in stems, and lowest in roots; values generally decreased as the plants grew. Data obtained will be of value in growing and sampling plants in future experiments.

² Formerly graduate assistant in Agronomy (now graduate student in the Agronomy Department, North Dakota State Univ.); Research Geneticist, Crops Research Division, ARS, USDA; and Professor of Agronmny, University of Nebraska, respectively.

Received for publication July 23, 1965.