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Significant differences in seedling agronomic and quantitative biochemical characters (total carbohydrates) were obtained between four stocks of the same long-time inbred line of maize.
Apparently, 3 different biotypes have evolved in the 4 stocks of the inbred Cl 7. The stocks from North Carolina and Arkansas have diverged widely from each other as well as from the Athens and Beltsville stocks. Athens and Beltsville stocks have remained similar to each other. In general, the stocks which had agronomic differences in a previous study also had seedling agronomic and biochemical differences in this study.
Hereditary variations reported herein may be attributed to relic heterozygosity, mutation, or a combination of the two. These causes and the significance of the variations are discussed in a previous paper (2).
Studies in the determination of heterogeneity between stocks of the same inbred line are not limited to field experiments. Results of this experiment show that seedling characters in the greenhouse and quantitative biochemical analysis in the laboratory may be used successfully in differentiating stocks within a long-time inbred line. Furthermore, this information should be of value in relating biochemical studies to both applied and basic genetical research.
2 Graduate Assistant (now at Yale University) and Professor of Plant Breeding. The writers gratefully acknowledge the help of J. H. Owen, G. M. Kozelnicky, L. S. Dure III, M. K. Hamdy, S. J. Rowan, and R. A. McCreery.
Received for publication July 2, 1965.
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