HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Skinner, D. Z. Search for Related Content PubMed Articles by Skinner, D. Z. Agricola Articles by Skinner, D. Z. Related Collections Crop Physiology & Metabolism Wheat Cell Biology & Molecular Genetics

CROP PHYSIOLOGY & METABOLISM

Phospholipase A₂ Activity during Cold Acclimation of Wheat

USDA-ARS and Crop and Soil Sciences Dep., Washington State Univ., Pullman, WA, 99164

^* Corresponding author (dzs{at}wsu.edu).

The temperature lethal to 50% of the plants (LT₅₀) and phospholipase A₂ (PLA₂) activity levels were investigated in three wheat (Triticum aestivum L.) lines that had been cold acclimated 0 to 5 wks at 3°C. In the cold-tolerant winter wheat ‘Eltan’, the level of PLA₂ activity increased significantly during the first week of cold acclimation, but activity did not increase until the second week of acclimation in cold-sensitive winter wheat Oregon Feed Wheat #5 and spring wheat ‘Louise’. The PLA₂ activity declined in all lines by the end of 4 wks of acclimation. The LT₅₀ decreased (greater freezing tolerance) during the time that PLA₂ levels were elevated and then increased concomitant with decreased PLA₂ activity. A chilling treatment of –5°C for 45 min resulted in elevated PLA₂ activity in nonacclimated Eltan, as well as in all three wheat lines that had been acclimated for 1 wk, but not in plants that had been acclimated for more than 1 wk. These results suggest that PLA₂ activity is involved in response to cold temperature in wheat, but the intrinsic level of freezing tolerance and the level of acclimation reached by the plants mediates its activity.

Abbreviations: ORFW, Oregon Feed Wheat #5 • PCR, polymerase chain reaction • PED6, N-((6-(2,4-dinitrophenyl) amino)hexanoyl)-2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt • PLA₂, phospholipase A₂ • PLD, phospholipase D