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CROP BREEDING & GENETICS

Molecular Mapping and Confirmation of QTLs Associated with Oleic Acid Content in N00-3350 Soybean

^a Center for Applied Genetic Technologies, 111 Riverbend Rd, Univ. of Georgia, Athens, GA 30605
^b USDA-ARS, North Carolina State Univ., 3127 Ligon St. PO Box 7631 Raleigh, NC 27695
^c current address, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401

^* Corresponding author (mjmonteros{at}noble.org).

The fatty acid composition of soybean [Glycine max (L.) Merr.] seed affects the flavor, nutritional value, and stability of the oil. Increasing oleic acid content in soybean oil would reduce the need for hydrogenation, a process that creates unhealthy trans fatty acids. The objective of this study was to map and confirm the areas of the soybean genome associated with oleic acid content from the soybean line N00-3350 (583 g kg^–1 oleic acid) using simple sequence repeat (SSR) markers. An F_2:3 population of 259 lines from the cross of G99-G725 x N00-3350 was used as a mapping population, and an F_2:3 population of 231 lines from the cross of G99-G3438 x N00-3350 was used for confirmation. Using single-factor analysis of variance, interval mapping, and composite interval mapping, six quantitative trait loci (QTLs) for oleic acid content were found on linkage groups LG-A1 (Satt211, R² = 4%), LG-D2 (Satt389, R² = 6%), LG-G (Satt394, R² = 13%), LG-G (Satt191, R² = 7%), LG-L (Satt418, R² = 9%), and LG-L (Satt561, R² = 25%) in the G99-G725 x N00–3350 population. All six QTLs for oleic acid were confirmed in the G99-3438 x N00-3350 population. The designations cqOle-001, cqOle-002, cqOle-003, cqOle-004, cqOle-005, and cqOle-006 have been assigned to these confirmed QTLs by the Soybean Genetics Committee. The identification of SSR markers linked to the oleic acid QTLs will facilitate the use of marker-assisted selection (MAS) in soybean breeding programs to increase the oleic acid content in soybean seed.

Abbreviations: CIM, composite interval mapping • FDA, U.S. Food and Drug Administration • LG, linkage group • LOD, logarithm of odds • MAS, marker-assisted selection • MG, maturity group • MLG-MR, multiple linkage group regression analysis • PCR, polymerase chain reaction • QTL, quantitative trait locus • SF-ANOVA • single-factor analysis of variance • SLG-MR • single linkage group multiple regression analysis • SNP, single nucleotide polymorphism • SSR, simple sequence repeat

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				E. Bachlava, R. E. Dewey, J. W. Burton, and A. J. Cardinal Mapping and Comparison of Quantitative Trait Loci for Oleic Acid Seed Content in Two Segregating Soybean Populations Crop Sci., March 17, 2009; 49(2): 433 - 442. [Abstract] [Full Text] [PDF]