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CROP BREEDING & GENETICS

Identification of QTL for Resistance to Sclerotinia Stem Rot in Soybean Plant Introduction 194639

^a Dep. of Crop Sciences, Univ. of Illinois
^b USDA-ARS and Dep. of Crop Sciences, Univ. of Illinois, 1101 W. Peabody Dr., Urbana, IL 61801. Trade and manufacturers' names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by the USDA implies no approval of the product to the exclusion of others that may also be suitable

^* Corresponding author (ghartman{at}uiuc.edu).

Sclerotinia stem rot of soybean [Glycine max (L.) Merr.], caused by Sclerotinia sclerotiorum (Lib.) de Bary, is a difficult disease to manage, although some gains have been made through breeding for quantitative resistance. The objective of the present study was to map quantitative trait loci (QTL) controlling partial resistance to Sclerotinia stem rot from the soybean plant introduction (PI) 194639. The resistance QTL were mapped in a population of 155 F_4:5 recombinant inbred lines (RILs) developed from the hybridization of the partially resistant parent, PI 194639, to the susceptible cultivar Merit. The population was evaluated for Sclerotinia stem rot resistance using a cut stem inoculation technique and was genotyped with 134 simple sequence repeat (SSR) markers. Broad-sense heritability of lesion length (LL) after inoculation with the cut stem technique in the population was 0.57. Two putative QTL-controlling LL were identified by composite interval mapping (CIM) and mapped to linkage groups (LGs) A2 and B2, with likelihood of odds scores of 5.6 and 3.5, respectively. The LG A2 QTL was linked to the marker Sat_138 and explained 12.1% of the phenotypic variation for LL. The LG B2 QTL was proximal to the marker Satt126 and explained 11.2% of the phenotypic variance. Two minor QTL also were mapped onto LGs K and L, explaining 5.5% of the total phenotypic variation. A multivariate model that included all significant QTL explained 27% of the observed phenotypic variation of LL. These results suggest that SSR markers associated with resistance QTL mapped in this study for Sclerotinia stem rot resistance may be useful for marker-assisted breeding programs in soybean.

Abbreviations: CIM, composite interval mapping • DAI, days after inoculation • LG, linkage group • LL, lesion length • LOD, likelihood of odds • PCR, polymerase chain reaction • PI, plant introduction • QTL, quantitative trait loci • RIL, recombinant inbred line • SSR, simple sequence repeat

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				B. Calla, T. Vuong, O. Radwan, G. L. Hartman, and S. J. Clough Gene Expression Profiling Soybean Stem Tissue Early Response to Sclerotinia sclerotiorum and In Silico Mapping in Relation to Resistance Markers The Plant Genome, July 1, 2009; 2(2): 149 - 166. [Abstract] [Full Text] [PDF]