HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Sun, Q.-B. Articles by Ge, X.-J. PubMed Articles by Sun, Q.-B. Articles by Ge, X.-J. Agricola Articles by Sun, Q.-B. Articles by Ge, X.-J. Related Collections Biofuels Plant Genetic Resources

PLANT GENETIC RESOURCES

SSR and AFLP Markers Reveal Low Genetic Diversity in the Biofuel Plant Jatropha curcas in China

^a South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
^b State Key Lab. of Biocontrol, School of Life Sciences, Sun Yat-Sen Univ., Guangzhou 510275, China

^* Corresponding author (xjge{at}scbg.ac.cn).

Globally, Jatropha curcas L. (Euphorbiaceae) holds much promise for producing biodiesel; it is cultivated in many tropical countries. In the South China Botanical Garden, a germplasm collection of J. curcas has been assembled on the basis of geographic location. However, this collection has not been characterized using molecular techniques associated with germplasm management and utilization. In this study, the genetic relationships of 58 J. curcas accessions were assessed based on simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) analyses. Seventeen microsatellite markers were developed using the FIASCO (Fast Isolation by AFLP of Sequences Containing repeats) protocol; only one SSR primer was polymorphic with two alleles. The seven AFLP primer combinations amplified 70 polymorphic loci in total, 14.3% of which were polymorphic. The clustering of genotypes based on the AFLP markers shows that the genetic diversity of J. curcas in Guizhou was notably different from the other samples.

Abbreviations: AFLP, amplified fragment length polymorphism • DAMD, directed amplification of minisatellite-region DNA • GS, genetic similarity • ISSR, inter–simple sequence repeat • PCR, polymerase chain reaction • PIC, polymorphism information content • RAPD, random amplified polymorphic DNA • SSR, simple sequence repeat • UPGMA, unweighted pair group method using arithmetic averages