HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Kolmer, J. A. Articles by Lagudah, E. S. PubMed Articles by Kolmer, J. A. Articles by Lagudah, E. S. Agricola Articles by Kolmer, J. A. Articles by Lagudah, E. S. Related Collections Germplasm Enhancement Wheat Plant Disease Crop Genetics

PLANT GENETIC RESOURCES

Analysis of the Lr34/Yr18 Rust Resistance Region in Wheat Germplasm

^a USDA-ARS Cereal Disease Laboratory and Dep. of Agronomy and Plant Genetics, Univ. of Minnesota, St. Paul, MN 55108
^b CIMMYT, Apdo. Postal 6-641, 06600, Mexico DF, Mexico
^c Campo Experimental Valle de México INIFAP, Apdo. Postal 10, 56230, Chapingo, Edo de México, Mexico
^d CSIRO Plant Industry, Canberra ACT 2601, Australia
^e DNRE, Horsham, VIC, Australia
^f NSW Dep. of Primary Industries, Wagga Wagga, NSW 2650, Australia
^g John Innes Centre, Norwich, UK
^h Univ. of Sydney-Plant Breeding Institute, Camden, NSW, Australia

^* Corresponding author (evans.lagudah{at}csiro.au).

The Lr34/Yr18 adult plant resistance gene contributes significantly to durable leaf rust (caused by Puccinia triticina Eriks.) resistance. Simple and robust molecular markers that enable early detection of Lr34/Yr18 are a major advancement in wheat (Triticum aestivum L.) breeding. An insertion/deletion size variant located at the csLV34 locus on chromosome 7D within an intron sequence of a sulfate transporter-like gene tightly linked to the Lr34/Yr18 dual rust resistance gene was used to examine a global collection of wheat cultivars, landraces, and D genome–containing diploid and polyploid species of wheat relatives. Two predominant allelic size variants, csLV34a and b, found among the wheat cultivars showed disparate variation in different wheat growing zones. A strong association was observed between the presence of Lr34/Yr18 and the csLV34b allele and wheat lines known to have Lr34/Yr18 that had the csLV34a allele were rare. All landraces with the exception of those from China were predominantly of the csLV34a type. Only one size variant, csLV34a, was detected among the diploid and polyploid D genome–containing species, indicating that csLV34b arose subsequent to hexaploid bread wheat synthesis. The lineage of the csLV34b allele associated with Lr34/Yr18 in modern wheat cultivars from North and South America, CIMMYT, Australia, and Russia was tracked back to the cultivars Mentana and Ardito developed in Italy by Nazareno Strampelli in the early 1900s. The robustness of the csLV34 marker in postulating the likely occurrence of Lr34/Yr18 across a wide range of wheat germplasm and its utility in wheat breeding was confirmed.

Abbreviations: APR, adult plant resistance • HRWW, hard red winter wheat • HRSW, hard red spring wheat • KUDS, kinase-U box domain • PCR, polymerase chain reaction • SRWW, soft red winter wheat