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Published online 20 May 2008
Published in Crop Sci 48:1203-1210 (2008)
© 2008 Crop Science Society of America
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Patterns of Diversity in Populations of the Turfgrass Pathogen Colletotrichum cereale as Revealed by Transposon Fingerprint Profiles

Jo Anne Croucha, Bernadette M. Glasheena, Wakar Uddinb, Bruce B. Clarkea and Bradley I. Hillmana,*

a Dep. of Plant Biology and Pathology, Rutgers Univ., New Brunswick, NJ 08901-8520
b Dep. of Plant Pathology, The Pennsylvania State Univ., University Park, PA 16802

* Corresponding author (hillman{at}aesop.rutgers.edu).

Anthracnose disease of cool-season turfgrasses, caused by the fungus Colletotrichum cereale, has recently emerged as one of the most significant pathogens of Poa annua. Here we investigated the utility of four repetitive transposable elements as molecular markers for the analysis of C. cereale populations. Southern blot hybridization analysis revealed lineage-specific polymorphisms and distribution patterns for these transposons. Comparative phylogenetic analysis of three nonrepetitive protein coding DNA sequences against the transposon restriction fragment length polymorphisms indicated that the transposon sequences have similar evolutionary histories to those found in the sampled C. cereale population, despite the alteration of several transposon copies by repeat-induced point mutation. The variability and ubiquity of the Ccret2A15 transposon in C. cereale genomes suggest that this element could be used as a reliable DNA marker to discriminate between lineages of the fungus, identify hybrid genotypes, and analyze genetic diversity in populations of this turfgrass pathogen.

Abbreviations: ITS, intergenic transcribed spacer • kb, kilobase • PCR, polymerase chain reaction • RAPD, randomly amplified polymorphic DNA • RFLP, restriction fragment length polymorphism • RIP, repeat-induced point

We thank Lisa Vaillancourt for providing the Colletotrichum graminicola and C. sublineolum cultures and the National Institute of Agrobiological Sciences Genebank of Ibaraki, Japan, for the C. falcatum cultures used in this study. This work was funded by grants from the Rutgers Center for Turfgrass Science to B.I.H. and B.B.C. and by the New Jersey Agricultural Experiment Station. We gratefully acknowledge financial support for J.A.C.'s graduate studies provided by a U.S. Environmental Protection Agency Science to Achieve Results (STAR) Graduate Fellowship, the Ralph Geiger Endowment, a Rutgers Excellence Fellowship, the Robert White-Stevens Fellowship, the Peter Selmer Loft Memorial Scholarship fund, and a Land Institute Natural Systems Agriculture Graduate Fellowship. Although the research described in this article has been funded in part by the USEPA's STAR fellowship program through grant FP-91652101, it has not been subjected to USEPA review and therefore does not necessarily reflect the views of the agency, and no official endorsement of any products or commercial services mentioned in this article should be inferred.

All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.

Received for publication August 2, 2007.


This article has been cited by other articles:


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J. A. Crouch, B. B. Clarke, J. F. White Jr., and B. I. Hillman
Systematic analysis of the falcate-spored graminicolous Colletotrichum and a description of six new species from warm-season grasses
Mycologia, September 1, 2009; 101(5): 717 - 732.
[Abstract] [Full Text] [PDF]

Home page
 MycologiaHome page
J. A. Crouch, B. B. Clarke, and B. I. Hillman
What is the value of ITS sequence data in Colletotrichum systematics and species diagnosis? A case study using the falcate-spored graminicolous Colletotrichum group
Mycologia, September 1, 2009; 101(5): 648 - 656.
[Abstract] [Full Text] [PDF]


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