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Published online 19 March 2008
Published in Crop Sci 48:651-655 (2008)
© 2008 Crop Science Society of America
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Micropropagation of Field-Grown Perennial Teosinte from Node Culture

J. Zalea,d,*, L. Ohnoutkováa,c,d, D. Westa, C. Samsa, J. Biggerstaffb and M. Le Puilb

a Dep. of Plant Sciences, Univ. of Tennessee, Rm. 252 Ellington, 2431 Joe Johnson Dr., Knoxville, TN 37996-4561
b Univ. of Tennessee, Center for Biomarker Analysis, 10515 Research Dr., Suite 300, Knoxville, TN 37932-2575
c present address: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Sokolovská 6, 77200 Olomouc, Czech Republic
d contributed equally to this work

* Corresponding author (jzale{at}utk.edu).

Propagation of perennial diploid teosinte, Zea diploperennis Iltis, Doebley and Guzmán (2n = 2x = 20), and tetraploid teosinte, Z. perennis (Hitchc.) Reeves and Magelsdorf (2n = 4x = 40), is limited by seed availability. For plants grown in the field, a photoperiod response delays flowering until fall, and seeds may not reach maturity. The objective of this study was to develop an in vitro micropropagation protocol for field grown teosinte. Shoot proliferation was induced from nodes split longitudinally and plated on Murashige and Skoog's (MS) medium supplemented with 5 µM 6-benzyl amino purine and 3% (w/v) sucrose. Both species possess a single axillary bud that generated multiple plants on division. Rooting of shoots was achieved in half-strength MS medium with and without the addition of 0.4 µM indole-3-butyric acid and 2% (w/v) sucrose. Nodes and younger branches of the tetraploid generated significantly more plants than those of the diploid.

Abbreviations: 2,4-D, 2,4-dichlorophenoxyacetic acid • BAP, 6-benzyl amino purine • IBA, indole-3-butyric acid • MS, Murashige and Skoog • NAA, napthaleneacetic acid • PPM, Plant Preservation Mixture • TIFF, tagged image file format

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Received for publication February 13, 2007.




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