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Development of a Coupling-Phase SCAR Marker Linked to the Ur-7 Rust Resistance Gene and Its Occurrence in Diverse Common Bean Lines

^a Dep. of Horticultural Sciences, Texas A&M Univ., Weslaco, TX 78596
^b Dep. of Plant Pathology, Univ. of Nebraska, Lincoln, NE 68583
^c Dep. of Agronomy & Horticulture, Univ. of Nebraska, Lincoln, NE 68583. This paper was published as paper no. 14593 Journal Series, Nebraska Agricultural Research Division. Research was conducted under Projects 20-036 and 20-042

^* Corresponding author (jsteadman1{at}unl.edu).

Bean rust, caused by Uromyces appendiculatus (Pers.:Pers.) Unger, is an important disease of common bean (Phaseolus vulgaris L.). A coupling-phase random amplified polymorphic DNA (RAPD) marker OAD12.550 previously was identified to be linked (no recombination) to Ur-7 of Middle American (MA) origin for specific rust resistance in the common bean cross of Great Northern (GN) 1140 x GN Nebr. #1. However, a sequence characterized amplified region (SCAR) marker for Ur-7 present in GN1140 has not been reported. Our objectives were to convert the RAPD marker OAD12.550 most tightly linked to Ur-7 to a SCAR marker SOAD12.537 for use as a marker-assisted selection tool, and survey the presence or absence of the SCAR marker SOAD12.537 in 90 MA and Andean bean genotypes for determining the genetic relationship of Ur-7 with Ur-6. The coupling-phase SCAR marker SOAD12.537 based on a specific forward (5'-AAGAGGGCGTGAGATCGTCG-3') and reverse (5'-AAGAGGGCGTCTTGAAGGTT-3') primer pair showed no recombination with Ur-7 in an F₂ population of the GN1140 x Nebr. #1 cross. The SCAR marker was also present in pinto US-5 from which the rust resistance of GN1140 was derived and in the closely related pinto US-14. The cosegregating SCAR marker identified MA pinto bean cultivars/lines Olathe, Bill Z, Apache, Montrose, BelDak-RR-1 and-2, and CO 12783 that have rust resistance gene Ur-6 and also have Ur-7, identified in earlier literature as Ur_c, due to presence of the marker. Other cultivars/lines with Ur-6 such as Weihing, Burke, Kodiak, Topaz, Golden Gate Wax, BelMiNeb 1–13, BelDakMi 1–23, and other Colorado breeding lines lack Ur-7 because of absence of the SCAR marker for the MA gene. This SCAR marker linked to Ur-7 on linkage group 11 of the core P. vulgaris linkage map can identify a phenotypically hidden resistance gene, and along with markers for other rust resistance genes, can be utilized to pyramid multiple genes for more durable rust resistance.

Abbreviations: GN, great northern • LG, linkage group • MA, Middle American • PCR, polymerase chain reaction • RAPD, random amplified polymorphic DNA • RIL, recombinant inbred line • SCAR, sequence characterized amplified region

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Received for publication June 27, 2007.