Crop Science Journal of Natural Resources and Life Sciences Education
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Published online 16 January 2008
Published in Crop Sci 48:262-268 (2008)
© 2008 Crop Science Society of America
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A PCR-based Marker for the Rsv1 Locus Conferring Resistance to Soybean Mosaic Virus

Ainong Shi, Pengyin Chen*, Cuiming Zheng, Anfu Hou and Bo Zhang

Dep. of Crop, Soil, and Environmental Sciences, Univ. of Arkansas, Fayetteville, AR 72701

* Corresponding author (pchen{at}uark.edu).

Soybean mosaic virus (SMV) is a highly destructive viral disease. Three loci with 12 alleles conferring SMV resistance have been identified in soybean [Glycine max (L.) Merr.]. The objective of this study was to develop polymerase chain reaction–based markers for detecting the candidate gene 3gG2 at the Rsv1 locus for SMV resistance in diverse soybean germplasm. A pair of DNA primers, Rsv1-f/r, was developed from the 3gG2 sequence. This primer pair produced a 341-bp DNA fragment specific for soybean cultivars containing Rsv1. The same fragment was confirmed in genotypes containing Rsv1 alleles including ‘Kwanggyo’ (Rsv1-k), ‘Marshall’ (Rsv1-m), ‘Ogden’ (Rsv1-t), PI 96983 (Rsv1), PI 507389 (Rsv1-n), ‘Raiden’ (Rsv1-r), and ‘Suweon 97’ (Rsv1-s). The Rsv1-f/r fragment was also amplified in genotypes with two-gene combinations (Rsv1Rsv3 and Rsv1Rsv4) but not in genotypes containing only Rsv3 or Rsv4 or in the susceptible genotypes. The primer pair was used to screen for Rsv1 alleles in 97 SMV resistant genotypes. Results showed that 55 genotypes contained the 3gG2 gene, while 17 genotypes carrying the Rsv1-y allele and additional 25 SMV-resistant genotypes carrying Rsv3 or Rsv4 were not amplified a product using the 3gG2 primer pair. Analysis of an F2 population derived from J05 (Rsv1Rsv3) x ‘Essex’ (rsv1rsv3) showed a cosegregation between the marker Rsv1-f/r and the Rsv1 allele. Rsv1-f/r was also shown to be linked to simple sequence repeat marker Satt114 on soybean molecular linkage group F with a distance of 5.42 cM. This marker will give breeders a tool for marker-assisted selection and gene pyramiding in soybean breeding for SMV resistance.

Abbreviations: MAS, marker-assisted selection • MLG, molecular linkage group • PCR, polymerase chain reaction • PI, plant introduction • RAPD, random amplification of polymorphic DNA • RFLP, restriction fragment length polymorphism • SMV, Soybean mosaic virus • SSR, simple sequence repeat.

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Received for publication February 10, 2007.




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