Crop Science Journal of Natural Resources and Life Sciences Education
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Published online 1 January 2007
Published in Crop Sci 47:S-60-S-67 (2007)
© 2007 Crop Science Society of America
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ACTIVITIES & RESOURCES

Getting the Point—Mutations in Maize

Clifford F. Weil* and Rita-Ann Monde

Dept. of Agronomy, Purdue University, 1150 Lilly Hall, 915 W. State St., West Lafayette, IN 47906

* Corresponding author (cweil{at}purdue.edu).

Point mutations are important tools for understanding gene functions and genetic interactions, as well as for identifying neomorphs. The Maize Targeting Induced Local Lesions IN Genomes (TILLING) Project has been established to provide reverse genetics resources that can screen ethyl methonyl sulfonate (EMS)–mutagenized populations of maize (Zea mays L.) for individuals carrying point mutations in virtually any gene in the genome. In addition, a variation on TILLING, EcoTILLING, can be used on Maize Diversity Lines to gauge how much genetic diversity is present in maize germplasm at any given locus. The maize populations developed for TILLING, in the B73 and W22 inbred lines, also serve as an excellent and publicly available forward genetics resource.







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