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CROP BREEDING & GENETICS

Genetic Mapping of a Triticum monococcum-derived Powdery Mildew Resistance Gene in Common Wheat

L. M. Miranda^a,*, L. Perugini^a, G. Srni^b, G. Brown-Guedira^c, D. Marshall^c, S. Leath^d and J. P. Murphy^a

^a Dep. of Crop Science, North Carolina State Univ., Raleigh, NC 27695
^b Pioneer Hi-Bred Italia, Via Giuseppina 39, Malagnino, Cremona 26030 Italy
^c USDA-ARS; North Carolina State Univ., Raleigh, NC 27695
^d Dep. of Plant Pathology, North Carolina State Univ., Raleigh, NC 27695

^* Corresponding author (lmiranda{at}uga.edu).

Powdery mildew of wheat (Triticum aestivum L.) is a major fungal disease caused by Blumeria graminis DC f. sp. tritici. A microsatellite linkage map was developed for the T. monococcum-derived powdery mildew resistant gene present in the North Carolina germplasm line NCBGT96A6 (NCA6). Genetic analysis of F₂-derived lines from the cross NCA6 x ‘Saluda’ indicated a single gene controlled powdery mildew resistance. Four microsatellite markers linked to the NCA6 Pm gene mapped to chromosome 7AL. The most likely order was Xcfa2123-0.9 cM–Xbarc121-1.7 cM resistance gene/Xcfa2019-3.0 cM-Xgwm332. A detached-leaf test indicated the disease reaction response of the NCA6 Pm gene was different from the five known alleles at the Pm1 locus on 7AL. Deletion interval mapping showed a large physical to genetic distance ratio for these microsatellite marker loci. This may be due to suppressed recombination between the introgressed T. monococcum segment and the homologous region of the T. aestivum cultivar Saluda. Our results suggested that the NCA6 Pm gene is likely a novel source of resistance to powdery mildew but additional allelism studies are needed to establish the relationship between this locus and the other known Pm loci on 7AL.

Abbreviations: LOD, decimal logarithm of odds • PCR, polymerase chain reaction • RAPD, random amplified polymorphic DNA