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GENOMICS, MOLECULAR GENETICS & BIOTECHNOLOGY

Marker-Assisted Selection of Columbia Root-Knot Nematode Resistance Introgressed from Solanum bulbocastanum

^a IAREC, Washington State Univ., 24106 N. Bunn Rd., Prosser, WA 99350
^b USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350
^c USDA-ARS and Univ. of California-Berkeley, Plant Gene Expression Center, 800 Buchanan St., Albany, CA 94710

^* Corresponding author (cbrown{at}pars.ars.usda.gov).

The Columbia root-knot nematode (Meloidogyne chitwoodi Golden et al.) is a serious pest that reduces tuber quality of potato (Solanum tuberosum L.) in the U.S. Northwest and other parts of the world. A gene, R_Mc1(blb), derived from the Mexican wild species Solanum bulbocastanum Dunal, encodes resistance to this pest. An F₁ mapping population with >250 individuals generated from an intraspecific cross between resistant and susceptible clones of S. bulbocastanum, SB22 and PT29, respectively, was used for marker screening and genetic linkage analysis. One amplified fragment length polymorphism marker and five sequence tagged site (STS) markers cosegregated with R_Mc1(blb). The five STS markers were developed from bacterial artificial chromosome (BAC) end sequences of BAC clones that were derived from another wild species, S. demissum Lindl, and contained homologs of resistance gene N against tobacco mosaic virus. These markers were tested on families that were part of the introgression of R_Mc1(blb) into advanced breeding lines in BC₅. The utility of an efficient alternative to greenhouse and field phenotypic screening was demonstrated. The results of this study confirm that molecular markers closely linked to R_Mc1(blb) will assist in a selection program, reducing expense and time involved in root-knot nematode screening.

Abbreviations: BAC, bacterial artificial chromosome • cM, centimorgan • CRN, Columbia root-knot nematode • PCR, polymerase chain reaction • RF, reproductive factor • STS, sequence tagged site