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PLANT GENETIC RESOURCES

Genetic Diversity of Canadian Soybean Cultivars and Exotic Germplasm Revealed by Simple Sequence Repeat Markers

^a Plant Gene Resources of Canada, Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Pl., Saskatoon, SK, Canada S7N 0X2
^b Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, K.W. Neatby Building, Ottawa, ON, Canada K1A 0C6

^* Corresponding author (fuy{at}agr.gc.ca).

Genetic diversity assessment of improved crop germplasm can facilitate the expansion of the genetic base in a plant breeding program, but little effort has been made to assess the Canadian soybean [Glycine max (L.) Merr.] gene pool established over the past century. Simple sequence repeat (SSR) markers were applied to assess the genetic diversity of 45 Canadian soybean cultivars released from 1934 to 2001 and 37 exotic germplasm accessions. Thirty-seven SSR primer pairs were applied and 234 polymorphic bands were scored for each accession. The frequencies of the scored bands ranged from 0.01 to 0.90 and averaged 0.17. The proportion of total SSR variation occurring between exotic and Canadian germplasm was 9%; among the Canadian cultivars of three breeding periods 10%; and between the cultivars of maturity groups 0 and 00 4%. More diversity was found for exotic germplasm than the Canadian. More diversity was observed in the cultivars of the recent breeding period than the early. The Canadian cultivars were clustered into seven major groups, partially congruent to the known pedigrees, and they were more related to germplasm from Russia, Sweden, and Ukraine and less to the Asian germplasm. The six genetically most distinct cultivars were PS86 RR, Gaillard, Manitoba Brown, Beechwood, Maple Isle, and 92B91. These findings are useful for the selection of genetically distinct or less related soybean materials to improve the genetic background of the soybean gene pool.

Abbreviations: AD, average dissimilarity • AMOVA, analysis of molecular variance • EST, expressed sequence tag • MG, maturity group • PCR, polymerase chain reaction • PGRC, Plant Gene Resources of Canada • PIC, polymorphic information content • SSR, simple sequence repeat