HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gao, Y. Articles by Buss, G. R. Search for Related Content PubMed Articles by Gao, Y. Articles by Buss, G. R. Agricola Articles by Gao, Y. Articles by Buss, G. R. Related Collections Crop Genetics Soybean Phosphorus

CROP BREEDING & GENETICS

A Modified Colorimetric Method for Phytic Acid Analysis in Soybean

^a Dep. of Crop and Soil Environmental Sciences, Virginia Tech., Blacksburg, VA, 24061
^b Dep. of Plant Pathology, Physiology and Weed Science, Virginia Tech., Blacksburg, VA, 24061
^c USDA-ARS and Crop Science Dep., North Carolina State Univ., Raleigh, NC 27695

^* Corresponding author (smaroof{at}vt.edu).

A quantitative, reproducible, and efficient phytic acid assay procedure is needed to screen breeding populations and support genetic studies in soybeans [Glycine max (L.) Merr.]. The objective of this study was to modify the colorimetric Wade reagent method and compare the accuracy and applicability of this new method in determining seed phytic acid content in soybean with three well-established phytic acid assay methods: anion exchange column (AEC), high-performance liquid chromatography (HPLC), and ³¹P nuclear magnetic resonance (NMR). The CV for repeated measurements of a low phytic acid soybean mutant, CX1834-1-6, ranged from 1.8 to 4.2% (n = 5), indicating the results were precise and reproducible. Phytic acid content of 42 soybean genotypes as determined by this method showed a correlation of 93.7 to 96.6% with the measurements by AEC, HPLC, and NMR. According to analysis of covariance, using inorganic P content as a predictor, phytic acid P content in a given sample analyzed by the four assay methods can be estimated with four linear regression models at the = 0.01 level. Compared with HPLC, AEC, and ³¹P NMR, this modified colorimetric method is simpler and less expensive for assaying a large number of samples, allowing its effective application in breeding and genetic studies of low phytic acid soybean.

Abbreviations: AEC, anion exchange column • ANCOVA, analysis of covariance • dd, distilled-deionized • EDTA, ethylenediaminetetraacetic acid • HPLC, high-performance liquid chromatography • ICP, inductively coupled plasma emission spectroscopy • IP, inositol phosphate • NMR, nuclear magnetic resonance • PA, phytic acid • Pi, inorganic phosphorus • QTL, quantitative trait loci

This article has been cited by other articles:

				Y. Gao, R.M. Biyashev, M.A. S. Maroof, N.M. Glover, D.M. Tucker, and G.R. Buss Validation of Low-Phytate QTLs and Evaluation of Seedling Emergence of Low-Phytate Soybeans Crop Sci., July 1, 2008; 48(4): 1355 - 1364. [Abstract] [Full Text] [PDF]