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CROP BREEDING & GENETICS

SSR Mapping and Confirmation of Soybean QTL from PI 437654 Conditioning Resistance to Reniform Nematode

^a Dep. of Crop and Soil Science, Univ. of Georgia, Center for Applied Genetic Technologies, 111 Riverbend Rd., Athens, GA 30602
^b Dep. of Plant Pathology, Univ. of Arkansas, Fayetteville, AR 72701
^c Pioneer Hi-Bred International, Inc., Johnston, IA 50131
^d Dep. of Plant Pathology, Univ. of Georgia, Athens, GA 30602

^* Corresponding author (ha{at}uga.edu).

Recently, reniform nematode (Rotylenchulus reniformis Linford and Oliveira) has been recognized to be a major problem on soybean [Glycine max (L.) Merr.] in the southern USA. Planting reniform nematode–resistant soybean cultivars is the most cost effective control method. With restriction fragment length polymorphism (RFLP) markers, Pioneer Hi-Bred International, Inc. had previously identified quantitative trait loci (QTL) on linkage group B1 (LG-B1) and another on LG-L associated with reniform nematode resistance in a cross between ‘BSR101’ and PI 437654. In this study, we refined the QTL locations and identified additional QTL conditioning resistance to reniform nematode in a population of 228 recombinant inbred lines (RILs) from a cross of BSR101 x PI 437654. A QTL (R² = 21%) conditioning reniform reproductive index (RI) was found on LG-L and was flanked by Sat_184 and Satt513. Two other QTL were identified, one on LG-B1 (R² = 16%) and the other on LG-G (R² = 8%). These two QTL acted in an epistatic manner with lines homozygous for PI 437654 alleles at both QTL providing the lowest reniform RI. The 31 RILs from BSR101 x PI 437654 that were homozygous for the PI 437654 alleles at Satt513, Satt359, and Sat_168 averaged 1077 reniform eggs and juveniles (RI = 0.63), while the 33 RILs homozygous for the BSR101 alleles averaged 10606 (RI = 6.24). By screening the RILs population of ‘Prichard’ x ‘Anand’ we confirmed that the QTL on LG-G and-B1 conditioned reniform RI.

Abbreviations: LG, linkage group • MAS, marker-assisted selection • NIL, near isogenic line • PCR, polymerase chain reaction • Pf, final population • Pi, initial inoculum level • QTL, quantitative trait loci • RFLP, restriction fragment length polymorphism • RI, reproductive index • RIL, recombinant inbred line • SCN, soybean cyst nematode • SSR, simple sequence repeat

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