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GENOMICS, MOLECULAR GENETICS & BIOTECHNOLOGY

DNA Marker Analysis of ‘Davis’ Soybean and Its Descendants for the Rcs3 Gene Conferring Resistance to Cercospora sojina

^a Center for Applied Genetic Technologies, The Univ. of Georgia, 111 Riverbend Rd., Athens GA 30602-6810
^b Dep. of Plant Pathology, Georgia Exp. Stn., Griffin, GA 30223

^* Corresponding author (rboerma{at}uga.edu).

Frogeye leaf spot (FLS), caused by Cercospora sojina Hara, is a serious disease of soybean [Glycine max (L.) Merr.]. Complete resistance to all isolates of FLS was reported in the cultivar Davis, and the Rcs3 gene was mapped on Linkage Group J (LGJ), near the simple sequence repeat (SSR) markers Satt244 (64.9 cM) and Satt547 (67.7 cM). The objectives of this study were to track the transmission of Rcs3 from Davis to its descendants and determine the cosegregation of Rcs3 and Davis SSR marker bands. Reaction of 64 lines inoculated with 15 virulent isolates of C. sojina was used in conjunction with molecular data from four SSR markers spanning 6.2 cM surrounding the Satt244–Satt547 interval. In addition, 38 putative soybean differentials were characterized for these markers and their reaction to 93 isolates of C. sojina to evaluate the uniqueness of the Rcs3 alleles. The results showed an association (Yules Y = 0.87, p < 0.001) between resistance to FLS and Satt244 and Satt547. However, unlike previously reported, the locus Satt244 is duplicated. Cosegregation of the 156/182-bp bands at Satt244 and 242-bp band at Satt547 in known Rcs3 lines suggests that the two markers can be used in the selection for resistance to FLS if the pedigree traces to Davis or one of its Rcs3 descendants. It also suggests the possibility of linkage disequilibrium in this segment of LGJ. Therefore, analyzing resistance to FLS with either one of the two SSR markers would be cost-effective in marker-assisted selection.

Abbreviations: FLS, frogeye leafspot • LGJ, Linkage Group J • SSR, simple sequence repeat.

This article has been cited by other articles:

				M. A. R. Mian, A. M. Missaoui, D. R. Walker, D. V. Phillips, and H. R. Boerma Frogeye Leaf Spot of Soybean: A Review and Proposed Race Designations for Isolates of Cercospora sojina Hara Crop Sci., January 16, 2008; 48(1): 14 - 24. [Abstract] [Full Text] [PDF]