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TURFGRASS SCIENCE

Antioxidant Responses of Creeping Bentgrass Roots to Waterlogging

Dep. of Agronomy, Purdue Univ., West Lafayette, IN 47907

^* Corresponding author (yjiang{at}purdue.edu)

Antioxidant enzymes protect plant cells from oxidative injury induced by hypoxia. The objective of this study was to identify the responses of antioxidant enzymes to different depths of waterlogging (WL) in creeping bentgrass (Agrostis stolonifera L.) roots. ‘Penncross’ and ‘G-6’ were subjected to 21 d of WL at 1 cm (WL-1) and 15 cm (WL-15) below the soil surface, respectively. The turf quality of Penncross was not acceptable under both WL conditions, while G-6 had an acceptable quality of 6.5 under WL-15. The activity of superoxide dismutase (SOD) increased by 32 and 26% for Penncross and 83 and 44% for G-6 under WL-15 and WL-1, respectively. One isoform of Mn-SOD and three isoforms of Cu/Zn-SOD were identified in both cultivars under all treatments. The activity of ascorbate peroxidase (AP) significantly decreased by 46 and 40% for Penncross, while AP activity decreased 25% and increased 9% for G-6 under WL-1 and WL-15, respectively. One isoform of AP was identified with strong intensity in both cultivars under all treatments. The content of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA, 1,1,3,3-tetramethoxypropane) and the activities of glutathione reductase (GR) and peroxidase (POD) were not affected by WL for both cultivars. These results indicated that SOD and AP were mainly involved in WL-induced antioxidant responses, and the partial WL could also significantly affect root antioxidant activities, particularly in WL-sensitive cultivar.

Abbreviations: AP, ascorbate peroxidase • DR, dehydroascorbate reductase • Eh, soil redox potential • GR, glutathione reductase • MDA, malondialdehyde • MR, monodehydroascorbate reductase • NADPH, nicotinamide adenine dinucleotide phosphate • PAGE, polyacrylamide gel electrophoresis • POD, peroxidase • PVC, polyvinylchloride • ROS, reactive oxygen species • SOD, superoxide dismutase • WL, waterlogging