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Published online 22 January 2007
Published in Crop Sci 47:207-211 (2007)
© 2007 Crop Science Society of America
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GENOMICS, MOLECULAR GENETICS & BIOTECHNOLOGY-NOTES

A Mitochondrial Repeat Specific Marker for Distinguishing Wild Abortive Type Cytoplasmic Male Sterile Rice Lines from their Cognate Isogenic Maintainer Lines

P. Rajendrakumar, A. K. Biswal, S. M. Balachandran, M. S. Ramesha, B. C. Viraktamath and R. M. Sundaram*

Biotechnology Lab., Crop Improvement Section, Directorate of Rice Research, Rajendranagar, Hyderabad–500 030, Andhra Pradesh, India

* Corresponding author (rms_28{at}rediffmail.com)

The realization of yield potential of rice (Oryza sativa L.) hybrids developed through the "three-line system" largely depends on genetic purity of parental lines. Cytoplasmic male sterile (CMS) lines often get contaminated with cognate isonuclear maintainer lines during multiplication. We report identification of a rice mitochondrial DNA sequence, possessing a repeat motif that is polymorphic between the wild abortive (WA)-CMS lines and their cognate isonuclear maintainer lines. We have designed a primer pair flanking the unique repeat and two fragments of different size were amplified in all WA-CMS and their maintainer lines through PCR. The PCR assay was able to precisely detect the impurities in a commercial seed lot of a popular CMS line, IR58025A. The possibility of utilizing this marker as a replacement for the morphology-based (grow-out test) GOT in accurate detection of contaminants in CMS seed stocks is discussed.

Abbreviations: CMS, cytoplasmic male sterile • GOT, grow-out test • RAPD, random amplified polymorphic DNA • RFLP, restriction fragment length polymorphism • SNPs, single nucleotide polymorphisms • SSRs, simple sequence repeats • WA, wild abortive






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