Crop Science Journal of Natural Resources and Life Sciences Education
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Published online 8 September 2006
Published in Crop Sci 46:2022-2032 (2006)
© 2006 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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GENOMICS, MOLECULAR GENETICS & BIOTECHNOLOGY

Transgenic Expression of Onion Leaf Lectin Gene in Indian Mustard Offers Protection against Aphid Colonization

Munshi Azad Hossain, Mrinal K. Maiti, Asitava Basu, Supriya Sen, Arnab K. Ghosh and Soumitra K. Sen*

IIT-BREF Biotek, Indian Institute of Technology, Kharagpur 721302, India

* Corresponding author (sk_sen55{at}yahoo.co.in)

Genes of three naturally occurring monocot mannose-binding lectins from snowdrop (Galanthus nivalis L. agglutinin, GNA), garlic (Allium sativum L. leaf agglutinin, ASAL), and onion (Allium cepa L. agglutinin, ACA) and a recombinant fusion lectin between ASAL and ACA genes were expressed in a bacterial system. The pure and active form of the recombinant lectin peptides were utilized for estimation of their sensitivity potential against feeding nymphs of mustard aphid [Lipaphis erysimi (Kaltenbach)], a major sap-sucking insect pest of Indian mustard [Brassica juncea (L.) Czern.], an oilseed crop. The artificial diet bioassay revealed that ACA and the fusion lectin contained higher toxicity potential than GNA and ASAL. Ectopic expression of these lectins in mustard plants confirmed their protective capacity on the development of the population of aphids on transgenic plants. Based on the strong possibilities that lectins originating from diverse sources would have differential insecticidal potential against different insects, deployment of the appropriate lectin gene figures as crucial in the transgenic approach to protect crop plants against sap-sucking insect pests.

Abbreviations: ACA, Allium cepa L. agglutinin • ASAL, Allium sativum L. leaf agglutinin • GNA, Galanthus nivalis L. agglutinin • MBP, maltose-binding protein • ORF, open reading frame






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