HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Nichols, D. M. Articles by Diers, B. W. Search for Related Content PubMed Articles by Nichols, D. M. Articles by Diers, B. W. Agricola Articles by Nichols, D. M. Articles by Diers, B. W. Related Collections Soybean Crop Genetics

CROP BREEDING, GENETICS & CYTOLOGY

Fine Mapping of a Seed Protein QTL on Soybean Linkage Group I and Its Correlated Effects on Agronomic Traits

^a Dep. of Crop Sciences, University of Illinois, Urbana, IL 61801
^b Plant Science Dep., South Dakota State University, Brookings, SD 57007
^c Chromatin, Inc., 60 Hazelwood Dr., Champaign, IL 61820
^d Dep. of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68583

^* Corresponding author (bdiers{at}uiuc.edu)

Soybean [Glycine max (L.) Merr.] is primarily grown as a source of protein and oil. A quantitative trait locus (QTL) controlling seed protein concentration was previously mapped to linkage group (LG) I of soybean. The objectives of this study were to fine map the QTL and to determine if additional recombination could reduce the inverse phenotypic relationship between seed protein concentration and yield and oil concentration. The fine mapping was done with two sets of backcross populations that were tested in the field and with genetic markers. These populations were developed by the introgression of a high protein allele from the Glycine soja Sieb and Zucc. plant introduction (PI) 468916 into the genetic background of the breeding line A81–356022. The first set (Set 1) included three populations of backcross-four (BC₄) lines, and the second set (Set 2) included four populations of BC₅ lines. The populations segregated for different segments of the genomic region where the QTL maps. Tests of the two sets of populations resulted in the localization of the QTL for protein and oil to a 3-cM interval between the simple sequence repeat (SSR) marker Satt239 and the amplified fragment length polymorphism (AFLP) marker ACG9b. The results from the agronomic trait evaluations were inconsistent, making it difficult to definitively conclude whether the protein QTL controls these other traits through pleiotropy.

Abbreviations: AFLP, amplified fragment length polymorphism • BC, backcross • BSA, bulked segregant analysis • cM, centimorgan • LG, linkage group • MG, maturity group • PI, plant introduction • PCR, polymerase chain reaction • QTL, quantitative trait loci • RFLP, restriction fragment length polymorphism • SSR, simple sequence repeat

This article has been cited by other articles:

				J. Burstin, P. Marget, M. Huart, A. Moessner, B. Mangin, C. Duchene, B. Desprez, N. Munier-Jolain, and G. Duc Developmental Genes Have Pleiotropic Effects on Plant Morphology and Source Capacity, Eventually Impacting on Seed Protein Content and Productivity in Pea Plant Physiology, June 1, 2007; 144(2): 768 - 781. [Abstract] [Full Text] [PDF]