HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Mosjidis, J. A. Articles by Klingler, K. A. Search for Related Content PubMed Articles by Mosjidis, J. A. Articles by Klingler, K. A. Agricola Articles by Mosjidis, J. A. Articles by Klingler, K. A.

PLANT GENETIC RESOURCES

Genetic Diversity in the Core Subset of the U.S. Red Clover Germplasm

Dep. of Agronomy and Soils, Alabama Agric. Experiment Station, Auburn Univ., Auburn, AL 36849-5412

^* Corresponding author (mosjija{at}auburn.edu)

Biochemical diversity present in accessions of the red clover (Trifolium pratense L.) U.S. core subset is unknown. Its isozyme characterization would facilitate the study of relationship among accessions and assist in the identification of unique accessions and duplicates and in the detection of genetic differences among accessions. This study was undertaken to assess genetic diversity in the core subset using the isozymes esterase, ß-glucosidase, phosphoglucomutase, peroxidase, diaphorase, phosphoglucoisomerase, and superoxide dismutase. Ten isozyme loci with 30 alleles were detected. All loci were polymorphic in at least one accession. Percentage polymorphic loci within populations ranged from 50 to 100% with an average of 77.5%. At the species level, the number of alleles per polymorphic locus was 3.00, and the effective number of alleles per locus was 1.81. Within accession averages were 2.75 and 1.66, respectively. The core subset had a large amount of genetic diversity. Most accessions were different from each other except PI 207972 and PI 251564, PI 217507, and PI 229799 that were alike. One accession, PI 235867, was not a red clover. Most accessions (56) were in Hardy-Weinberg equilibrium, and thus they are more likely to maintain genotypic and allelic frequencies when increased. Genetic diversity at the species level was high and there was nearly twice as much variability among the wild populations as among the cultivars or landraces included in the core subset.