HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Colton, L. M. Articles by Jiang, J. Search for Related Content PubMed Articles by Colton, L. M. Articles by Jiang, J. Agricola Articles by Colton, L. M. Articles by Jiang, J. Related Collections Potato Plant Disease

CROP BREEDING, GENETICS & CYTOLOGY

Marker-Assisted Selection for the Broad-Spectrum Potato Late Blight Resistance Conferred by Gene RB Derived from a Wild Potato Species

Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA

^* Corresponding author (jjiang1{at}wisc.edu)

Potato (Solanum tuberosum L.) late blight, caused by Phytophthora infestans (Mont.) de Bary, is one of the most damaging diseases in any crop. Deployment of resistant varieties is the most effective way to control this disease. However, breeding for late blight resistance has been a challenge because the race-specific resistance genes introgressed from wild potato S. demissum Lindl. have been short lived and breeding for "horizontal" or durable resistance has achieved only moderate successes. We previously demonstrated that the high-level late blight resistance in a wild potato relative, S. bulbocastanum Dunal subsp. bulbocastanum, is mainly controlled by a single resistance gene RB. Transgenic potato lines containing the RB gene have showed strong late blight resistance, comparable to the backcrossed progenies derived from the somatic hybrids between potato and S. bulbocastanum. Here we report the development of a polymerase chain reaction-based DNA marker for tracking the RB gene in breeding populations derived from the potato x S. bulbocastanum somatic hybrids. Several marker-positive breeding lines showed the expected late blight resistance in greenhouse evaluations. Our results demonstrate that marker-based selection will allow us to effectively transfer the RB gene into potato using traditional breeding methods, an alternative to deploying the RB gene through genetic transformation.

Abbreviations: GM, genetically modified • kb, kilobase • LRR, leucine-rich repeat • PCR, polymerase chain reaction • RGA, resistance gene analog

This article has been cited by other articles:

				J. C. Kuhl, K. Zarka, J. Coombs, W. W. Kirk, and D. S. Douches Late Blight Resistance of RB Transgenic Potato Lines J. Amer. Soc. Hort. Sci., November 1, 2007; 132(6): 783 - 789. [Abstract] [Full Text] [PDF]

				L.-H. Zhang, H. Mojtahedi, H. Kuang, B. Baker, and C. R. Brown Marker-Assisted Selection of Columbia Root-Knot Nematode Resistance Introgressed from Solanum bulbocastanum Crop Sci., September 1, 2007; 47(5): 2021 - 2026. [Abstract] [Full Text] [PDF]