HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Free Full Text (PDF) Free Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (14) Citing Articles via Google Scholar Google Scholar Articles by Beebe, S. E. Articles by Lynch, J. P. Search for Related Content PubMed Articles by Beebe, S. E. Articles by Lynch, J. P. Agricola Articles by Beebe, S. E. Articles by Lynch, J. P. Related Collections Other Legumes Plant Nutrition Root Development Phosphorus

GENOMICS, MOLECULAR GENETICS & BIOTECHNOLOGY

Quantitative Trait Loci for Root Architecture Traits Correlated with Phosphorus Acquisition in Common Bean

^a Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali, Colombia
^b South China Agricultural Univ., Guangzhou, PRC
^c Univ. of Nebraska, Lincoln, NE, USA
^d Univ. of Florida, Hastings, FL, USA
^e Pennsylvania State Univ., University Park, PA, USA

^* Corresponding author (m.blair{at}cgiar.org)

Low soil P availability is a primary constraint to common bean (Phaseolus vulgaris L.) production in Latin America and Africa. Substantial genotypic variation in bean adaptation to low phosphorus (LP) availability has been linked with root traits that enhance the efficiency of soil foraging. The objectives of this study were to identify quantitative trait loci (QTLs) for P accumulation and associated root architectural traits, to facilitate genetic improvement and to reveal physiological relationships. Eighty-six F_5.7 recombinant inbred lines (RILs) were developed from a cross between G19833, an Andean landrace with high total P accumulation, and DOR 364, a Mesoamerican cultivar with low total P accumulation in LP conditions. A genetic map constructed with restriction fragment length polymorphisms (RFLPs), microsatellites, and PCR-based markers covering 1703 centimorgans (cM) total genetic distance and all eleven linkage groups (LGs) was used for QTL analysis. Seventy-one RILs were evaluated in the field at high phosphorus (HP) and LP for P accumulation, total root length (RL), specific RL, and plant dry weight (DW), while all 86 RILs were evaluated in a hydroponic system in the greenhouse for tap, basal, total, and specific RL and plant DW. Phosphorus accumulation in the field correlated with root parameters measured in the greenhouse. A total of 26 individual QTLs were identified for P accumulation and associated root characters using composite interval mapping (CIM) analysis. Phosphorus accumulation QTLs often coincided with those for basal root development, thus, basal roots appear to be important in P acquisition. Independent QTLs were identified for basal and taproot development, and for specific RL. Distinct QTLs for greater specific RL had positive, null and negative effects on P accumulation. Our results confirm the importance of root structure for LP adaptation and highlight the need for a more detailed understanding of root architectural traits for phenotypic as well as marker-aided selection of more P-efficient crops.

Abbreviations: AFLP, amplified fragment length polymorphism • CIM, composite interval mapping • cM, centimorgan • DW, dry weight • HP, high phosphorus • LG, linkage group • LOD, base 10 algorithm of the likelihood ratio • LP, low phosphorus • QTL, quantitative trait locus • R², proportion of variance explained by QTL at test site • RL, root length • SCAR, sequence characterized amplified region • RAPD, randomly amplified polymorphic DNA • RFLP, restriction fragment length polymorphism • RIL, recombinant inbred line • TR², proportion of variance explained for the QTL and the background markers • TSP, triple super phosphate

This article has been cited by other articles:

				N. C. Collins, F. Tardieu, and R. Tuberosa Quantitative Trait Loci and Crop Performance under Abiotic Stress: Where Do We Stand? Plant Physiology, June 1, 2008; 147(2): 469 - 486. [Full Text] [PDF]

				A. Fita, B. Pico, A. J. Monforte, and F. Nuez Genetics of Root System Architecture Using Near-isogenic Lines of Melon J. Amer. Soc. Hort. Sci., May 1, 2008; 133(3): 448 - 458. [Abstract] [Full Text] [PDF]

				M. D. Casler, D. J. Undersander, and W. E. Jokela Divergent Selection for Phosphorus Concentration in Reed Canarygrass Crop Sci., January 16, 2008; 48(1): 119 - 126. [Abstract] [Full Text] [PDF]

				J. J. Maxwell, M. A. Brick, P. F. Byrne, H. F. Schwartz, X. Shan, J. B. Ogg, and R. A. Hensen Quantitative Trait Loci Linked to White Mold Resistance in Common Bean Crop Sci., November 7, 2007; 47(6): 2285 - 2294. [Abstract] [Full Text] [PDF]

				M. Tesfaye, J. Liu, D. L. Allan, and C. P. Vance Genomic and Genetic Control of Phosphate Stress in Legumes Plant Physiology, June 1, 2007; 144(2): 594 - 603. [Full Text] [PDF]

				G. Hernandez, M. Ramirez, O. Valdes-Lopez, M. Tesfaye, M. A. Graham, T. Czechowski, A. Schlereth, M. Wandrey, A. Erban, F. Cheung, et al. Phosphorus Stress in Common Bean: Root Transcript and Metabolic Responses Plant Physiology, June 1, 2007; 144(2): 752 - 767. [Abstract] [Full Text] [PDF]