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GENOMICS, MOLECULAR GENETICS & BIOTECHNOLOGY

Expanding the Pool of PCR-Based Markers for Oat

Dep. of Agronomy, Iowa State Univ., 1208 Agronomy Hall, Ames, IA 50011-1010

^* Corresponding author (jjannink{at}iastate.edu)

Polymerase chain reaction (PCR)–based markers are generally more rapid and less expensive to assay than hybridization-based markers (e.g., restriction fragment length polymorphisms [RFLP]), making them useful for breeding applications, but few of such markers are available for oat (Avena sativa L.). Approaches to develop new markers cheaply include using primer pairs designed for other species or using publicly available sequence information. In this study we report on the design of 32 markers using publicly available oat sequence data and on the map locations of 20 loci from 16 markers on the oat ‘Ogle’ x ‘TAM O-301’ population.

Abbreviations: KO, ‘Kanota’ x ‘Ogle’ • OT, ‘Ogle’ x ‘TAM O-301’ • PCR, polymerase chain reaction • RFLP, restriction fragment length polymorphism • RIL, recombinant inbred line • SSR, simple sequence repeat • STS, sequence-tagged site

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