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CROP BREEDING, GENETICS & CYTOLOGY

Near-Infrared Transmission and Reflectance Spectroscopy for the Determination of Dietary Fiber in Barley Cultivars

^a II, USDA-ARS-RRC, P.O. Box 5677, Athens, GA 30604-5677
^b National Food Research Institute, Incorporated Administrative Agency, 2-1-12 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan

^* Corresponding author (sekays{at}qaru.ars.usda.gov)

Dietary fiber is an important quality parameter of barley (Hordeum vulgare L.) but is extremely laborious to measure. Near-infrared (NIR) transmission and reflectance spectroscopy were investigated as rapid screening tools to evaluate the total dietary fiber content of barley cultivars. The Foss Grainspec Rice Analyzer and NIR Systems 6500 spectrometer were used to obtain transmission and reflectance spectra, respectively, of polished grains and ground barley. Total dietary fiber was determined for each cultivar by AOAC Method 991.43. Modified PLS models developed for predicting total dietary fiber, using transmission spectra (850–1048 nm) of polished grains, had a standard error of cross validation (SECV) of 10.4 (range 58–197) g kg^–1 and R² of 0.82 indicating sufficient accuracy for selecting or rejecting high dietary fiber cultivars. NIR reflectance spectroscopy (1104–2494 nm) of ground barley samples resulted in a model with SECV of 5.2 (range 58–197) g kg^–1 and R² 0.96, indicating a high degree of precision in the prediction of total dietary fiber. The increased accuracy of the reflectance model may be due in part to more information available in the wavelength region used. The precision, low cost per sample and speed of measurement of the technique allow making dietary fiber selection decisions for large numbers of progeny in barley breeding programs.

Abbreviations: NIR, near-infrared • PLS, partial least squares • R², coefficient of determination • r², multiple coefficient of determination • SD, standard deviation • SEC, standard error of calibration • SECV, standard error of cross validation • TDF, total dietary fiber

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Crop Science 2005 45: vii. [Full Text]